ISURA, in collaboration with the Technical University of Braunschweig, recently published an article in the journal – Plants, on herbal authentication using a combination of chemical and biological test methods. The article showcases ISURA’s capabilities in the areas of morphological identification, HPTLC fingerprinting, advanced mass spectrometry, and DNA sequencing.
The published test methods will help find adulterants undetectable by traditional means such as HPTLC and morphological identification. Furthermore, the data has been submitted to the National Center for Biotechnology Information (NCBI) to prevent future misidentification of horsetail plants through DNA sequencing.
A Multi-Pronged Technique for Identifying Equisetum palustre and Equisetum arvense—Combining HPTLC, HPLC-ESI-MS/MS and Optimized DNA Barcoding Techniques
Plants 2022, 11(19), 2562; https://doi.org/10.3390/plants11192562
The most prominent horsetail species, Equisetum arvense, has an array of different medicinal properties, thus the proper authentication and differentiation of the plant from the more toxic Equisetum palustre is important. This study sought to identify different samples of E. arvense and E. palustre using three analytical methods. The first method involved the use of HPTLC analysis, as proposed by the European Pharmacopoeia. The second, HPLC-ESI-MS/MS, is capable of both identification and quantification and was used to determine the Equisetum alkaloid content in each sample. A third method was DNA barcoding, which identifies the samples based on their genetic make-up. Both HPTLC and HPLC-ESI-MS/MS proved to be suitable methods of identification, with HPLC-ESI-MS/MS proving the more sophisticated method for the quantification of alkaloids in the Equisetum samples and for determining the adulteration of E. arvense. For DNA barcoding, optimal primer pairs were elucidated to allow for the combined use of the rbcL and ITS markers to accurately identify each species. As new DNA marker sequences were added to GenBank, the reference library has been enriched for future work with these horsetail species.
Direct Link to Abstract in Plants: https://www.mdpi.com/2223-7747/11/19/2562